Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used.  Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science.  Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.

InhaltsangabePart I. Overview 1. An Overview of CE in Clinical Analysis            David S. Hage Part II. Applications in Clinical Chemistry 2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection            Petr Tuma and Karel Stulik 3. The Selective Determination of Sulfates, Sulfonates and Phosphates in Urine by Capillary Electrophoresis - Mass Spectrometry            Svenja-Catharina Bunz, and Christian Neusüß 4. Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids            Angelo Zinellu, Salvatore Sotgia, Luca Deiana, and Ciriaco Carru 5. Expanded Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)            Philip Britz-McKibbin 6. Development of an On-Site Measurement System for Salivary Stress-Related Substances Based on Microchip CE            Yoshihide Tanaka and Nahoko Naruishi 7. Simultaneous Determination of Atenolol and Amiloride by CZE with Capacitively Coupled Conductivity Detection (C4D)            Khaldun M. AL Azzam and Hassan Y. Aboul-Enein 8. A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Detection            Shulin Zhao and Yi-Ming Liu 9. High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems            Dieter Vanderschaeghe, Andras Guttman, and Nico Callewaert 10. Screening of Matrix Metalloproteinase Inhibitors by Microanalysis with Fluorescence Detection            Xin Hai, Erwin Adams, and Ann Van Schepdael Part III. Applications in Hematology 11. ABO Genotyping by Capillary Electrophoresis            James Chun-I Lee, Hsing-Mei Hsieh, Hsiao-Feng Teng, and Li-Chin Tsai 12. Separation of Hemoglobin Variants by Capillary Electrophoresis            Frédéric Cotton and Béatric Gulbis 13. Newborn Screening for Hemoglobinopathies Using Capillary Electrophoresis            P.C. Giordano Part IV. Applications in Bacteriology and Virology 14. Application of CZE in the Differentiation of Staphylococcus Aureus Strains            Boguslaw Buszewski, Katarzyna Hrynkiewicz, and Ewelina Dziubakiewicz 15. Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single Strand Conformation Polymorphism            Hee Sung Hwang, Gi Won Shin, Boram Chung, Jeongkyeong Na, and Gyoo Yeol Jung 16. Application of CGE to Virus Identification            Julia A. Fruetel and Victoria A. VanderNoot Part V. Applications in Disease Biomarker Discovery 17. Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery             Xueping Fang, Chenchen Wang, and Cheng S. Lee 18. Integrated Affinity and Electrophoresis Systems for Multiplexed Biomarker Analysis            Pamela N. Nge, Jayson V. Pagaduan, Weichun Yang, and Adam T. Woolley 19. Urinary Proteomics Based on Capillary Electrophoresis Coupled to Mass Spectrometry in Kidney Disease            Amaya Albalat, Julia Franke, Julien Gonzalez, Harald Mischak, and Petra Zürbig 20. Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as Atherothrombosis Biomarker            Angel Puerta, Pedro J. Martin-Alvarez, Sara Ongay, Jose C. Diez-Masa and  Mercedes de Frutos Part VI. Applications in Immunology 21. Chip-Based Immunoassays            Akwasi A. Apori and Amy E. Herr 22. CE Analys